Journal: Nature protocols
Article Title: Hydrodynamic size-based separation and characterization of protein aggregates from total cell lysates
doi: 10.1038/nprot.2015.009
Figure Lengend Snippet: HF5 separation device setup and flow parameters. (a,b) View of the HF5 double-length cartridge separation device and flow parameters for the sample focus step (a) and detailed view of the focus process inside the separation device (b). (c) Sample elution step: flow parameters. (d) View of the commercial HF5 cartridge—patent no. DE102010043877B3 (Superon). (e) Schematic of the instrumental setup required for the protocol depicting HPLC components (degasser, pump, autosampler and DAD) connected to the FFF separation system (Eclipse DUALTEC module) that hosts the HF5 device and the MALS (DAWN EOS used in this protocol). The PC controls all the instruments through specific software and performs data processing. DAD, diode array detector; FFF, field-flow fractionation; HF5, hollow-fiber flow FFF; MALS, multiangle light-scattering detector; VWD, variable wavelength detector.
Article Snippet: We thank Superon for the loan of the Eclipse DUALTEC flow FFF separation system and Eclipse ISIS software, and Wyatt Technology for the loan of the MALS detector DAWN EOS and Astra software.
Techniques: Software, Field Flow Fractionation, Multi-Angle Light Scattering